1. Clean vial stopper with alcohol swab.
2. Fill syringe with equal amount of air in comparison to the intended dose.
3. Once alcohol is dry, insert 20 g needle into vial.
4. Inject the air into the vial. This keeps a balance of internal/external pressure within the vial.
5. Draw desired amount of solution into syringe and remove syringe from vial.
6. Holding the needle end of syringe upright, tap the side of the syringe, and expel any extra air bubbles (tiny bubbles ARE NOT a danger to health, but this is still a correct practice).
7. Clean the injection site with an alcohol swab with a circular motion starting at the injection site and moving outward to create a 2 inch diameter sterile area on skin.
8. Switch from 20 g needle to 25 g injection needle.
9. Stretch the skin over the site of the injection with the thumb and forefinger of your free hand, and penetrate the muscle with the needle. If you hit a nerve, and you’ll know if you do, extract needle and penetrate in another position.
10. Pull back on the stopper a tiny bit until you see either an air bubble or blood enter the syringe. If you see a bubble, you have a green light for injection. If you see blood, extract the needle and start back at step 9 (this is to avoid injecting into a blood vessel).
11. Press the stopper down firmly and steadily until all of the solution has been injected. Wait 5 seconds and remove the needle. Then press down on the injection site with a paper towel or toilet paper. While pressing down start to slowly massage the site for a minute or so. If area is still bleeding, continue to keep pressure until the bleeding stops.
12. Dispose of the needles and syringe properly.
Below are diagrams of the 3 main injection sites used. You can also use your biceps, triceps, and chest, although they are more difficult, especially the chest, so I wouldn't recommend using these sites unless you absolutely have to.
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